Journal: Cell Discovery
Article Title: LncRNA TubAR complexes with TUBB4A and TUBA1A to promote microtubule assembly and maintain myelination
doi: 10.1038/s41421-024-00667-y
Figure Lengend Snippet: a Illustration of rotarod behavioral test shown in b , k . b Rotarod test results from shCtrl AAV ( n = 5) and sh TubAR -1 AAV ( n = 5) secondary motor cortex-injected mice. c Schematic illustration of MRI analysis. d MRI images of Slice 8 (vermis) in four shCtrl AAV and four sh TubAR -1 AAV mice. Arrows indicate areas with altered MRI signals between shCtrl AAV and sh TubAR -1 AAV mice. e Schematic illustration of the microscopic analytic region for f – q . f , h , n , p Representative immunostaining images of CALBINDIN ( f ), CNP ( h ), NeuN ( n ), and vGLUT1 ( p ) in the shCtrl AAV and sh TubAR s AAV mice at cerebellar cortex region. Slice thickness, 40 µm. g , i , o , q Quantification of CALBINDIN, CNP, NeuN, and vGLUT1 using ImageJ in f , h , n , p ( n = 3). j , l Representative immunostaining images of NFL ( j ) and CNP ( l ) in the shCtrl AAV and sh TubAR s AAV mice at white matter region. Slice thickness, 40 µm. k , m Quantification of NFL and CNP using ImageJ in j , l ( n = 3). r Rotarod test results from shCtrl AAV ( n = 36), sh TubAR -1 AAV ( n = 39) and sh TubAR -2 AAV ( n = 13) cerebellum-injected mice. s Illustration of open-field behavioral test shown in t , u . t , u Total movement distance ( t , left), representative recorded routes ( t , right) and velocity of active movement ( u ) of open-field test from shCtrl AAV ( n = 10), sh TubAR -1 AAV ( n = 10), and sh TubAR -2 AAV ( n = 10) cerebellum-injected mice. Data are represented as means ± SEM. Statistical significance was determined using unpaired t -test with Welch’s correction ( g , i , k , m , o , q ) or mixed-effects model with Geisser–Greenhouse correction ( b , r ); ns, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001. AAV adeno-associated virus, ML molecular layer, PCL Purkinje cell layer, GCL granule cell layer, WM white matter, PC Purkinje cell.
Article Snippet: The primary antibodies used were chicken anti-NFL (Abcam, Cat#ab72997, RRID: AB_1267598, 1:300), rabbit anti-CALBINDIN (Abcam, Cat#ab108404, RRID: AB_10861236, 1:100), mouse anti-CNP (Abcam, Cat#ab6319, RRID: AB_2082593, 1:300), rabbit anti-NeuN (Abcam, Cat#ab177487, RRID: AB_2532109, 1:300), or rabbit anti-vGLUT1 (Abcam, Cat#ab227805, RRID: AB_2868428, 1:100).
Techniques: Injection, Immunostaining, Virus
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